Activity Stained Gel

A nondenaturing 4–30% gradient gel was stained for HuBChE activity by the method of Karnovsky and Roots,¹⁴ as shown in Figure ​Figure44.

Low HuBChE activity in unfolded HuBChE. The nondenaturing gradient gel in panel A stained with Coomassie blue was loaded with 4.4 μg HuBChE protein per lane. The gel in panel B stained for HuBChE activity was loaded with 0.005 units of HuBChE activity, which required 0.01 μg of HuBChE protein for the 4 °C sample and 0.04 μg HuBChE protein for the 45 °C sample. The 100 °C sample had no activity.