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2.6. β‐galactosidase (SA‐β‐gal) staining
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In brief, the treated SH‐SY5Y neuronal cells were washed with PBS buffer and then fixed with 2% paraformaldehyde at room temperature for 5 min. Subsequently, the cells were incubated with fresh SA‐β‐gal stain solution at 37°C. Lastly, after being photographed, the percentage of SA‐β‐gal positive perinuclear blue cells was recorded.
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