2.5. SFK kinase activity assay

A Takara Universal Tyrosine Kinase Assay Kit (Clontech Laboratory, Inc., Mountain View, CA) was used to assess SFK kinase activity as described previously (D. Z. Liu et al., 2014; Mao et al., 2018). Briefly, we used a mouse antibody against Fyn or Src to immunoprecipitate a protein of interest from striatal or mPFC homogenates. Immunopurified Fyn or Src proteins and ATP were added to microplate wells that were covered with an immobilized tyrosine kinase substrate poly (Glu‐Tyr) at 37°C for 30 min. Wells were washed and blocked. A horseradish peroxidase–conjugated anti‐phosphotyrosine antibody was added into wells, followed by measurement of phosphorylated substrates in absorbance at 450 nm.