acRIP-seq

acRIP-seq was conducted in two groups of BMSCs (two biological repeats per group), including BMSCs cultured in OM with siNC and OM with siNAT10 on day 7. Total RNA was collected using the method described above. Total RNA was heated to 70°C for 6 min. Then EDTA was used to stop the reaction. The fragmented RNA was purified and collected by a Zymo RNA Clean and Concentrator-25 kit (R1017; Zymo Research). An anti-ac4C antibody, Dynabeads Protein G (10004D; Invitrogen), and purified RNA were incubated at 4°C for 6 h. The immunoprecipitated RNA was collected according to the manufacturer’s instructions. The library was constructed using an EpiTM Mini LongRNA-seq Kit (E1802; Epibiotek) according to the manufacturer’s protocol. Quality control of the library was conducted with a Bioptic Qsep100 Analyzer (Bioptic). NovaSeq, a high-throughput sequencing platform, was used for sequencing by Epibiotek (Guangzhou, China).