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SLAMF1 surface staining and fluorescence-activated cell sorter (FACS) analysis.
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For cell surface staining for SLAMF1 (CD150), 1 × 106 cells were first blocked with 1 μg of human IgG and incubated with 1 μg of anti-CD150 phycoerythrin (PE)-conjugated (number 12-1502; eBioscience, San Diego, CA) antibodies on ice. Cells were then fixed and permeabilized with Cytofix/Cytoperm solution (number 554722; BD Biosciences, San Jose, CA). All flow cytometry data were acquired on a BD FACSVerse (BD Biosciences) and were analyzed using FlowJo software (Tree Star Inc.).
Copyright and License information: ©2016, American Society for Microbiology. All Rights Reserved.
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