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For cell counting assays, an equal number of cells was seeded in a 12-well plate 24 h before the proliferation assay. The cell number in each well was counted daily by using the Z1 Particle Counter (Beckman Coulter) for 4–5 d. The BrdU assay (Roche) was performed based on the manufacturer’s instructions. Data were from three independent experiments and are presented as means ± SD.
Copyright and License information: ©2016
Freely available online through the PNAS open access option.
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