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Transcriptome sequencing was performed in 16 cases of HCC tissues and paired NT liver tissues. TruSeq Standard mRNA Sample Prep Kit (Illumina) was used to prepare the polyA + mRNA library. We performed 100-bp paired-end sequencing in Illumina HiSeq2000 by Axeq Technologies. Data were analyzed by the TopHat-Cufflinks pipeline (37), and values were indicated by FPKM.
Copyright and License information: ©2016
Freely available online through the PNAS open access option.
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