2.11. Evaluation of the Locomotive Function

To evaluate the locomotive function of hindlimbs, BMS (Basso Mouse Scale) system was used before surgery and 1, 3, 7, 14, 21 and 28 days post-SCI [33]. The BMS ranges from 0 (complete paralysis) to 9 (normal locomotion) points. The 11-point BMS subscore which included frequency of plantar stepping, coordination, paw position, trunk stability, and tail position was also assessed and supplemented the main scale. Each mouse was observed 5 min, and the average BMS scores and subscores were recorded by two well-trained investigators who were familiar with the BMS scores and blinded to the experimental design.