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DSB Repair by Ectopic Recombination
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DSB repair by ectopic recombination was detected by using the tGI354 strain as described in [72]. To determine the repair efficiency, the intensity of the uncut band at 2 h after HO induction (maximum efficiency of DSB formation), normalized respect to a loading control, was subtracted to the normalized values of NCO and CO bands at the subsequent time points after galactose addition. The obtained values were divided by the normalized intensity of the uncut MATa band at time zero before HO induction (100%).
Copyright and License information: ©2016 Cassani et al
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