Sequence and gene expression information

The ORFs and intron-containing genes sequence information for S. cerevisiae (strain 288C) was taken from SGD [28] and the Ares lab database [29]. S. pombe genome information was taken from the PomBase database (Assembly 16) [30] and the original full genome sequencing obtained by [31]. The genome of E. coli (K-12, MG1655) was downloaded from the NCBI website (https://www.ncbi.nlm.nih.gov/). The protein abundance (PA) information for all organisms was taken from PaxDb [19]. The mRNA levels for S. cerevisiae were obtained by integration of the following data sets: [20, 32, 33]. Levels of mRNA for E. coli were taken from [34]. The mRNA levels for S. pombe are based on [35]. See full details in [36].