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Analysis of the expression of PD-1 and NKG2D by flow cytometry
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CD8+ T and NK cells were resuspended at a density of 1 × 105 cells/mL, and 20 μL APC anti-human NKG2D (BD, USA) or FITC anti-human PD-1(BD, USA) was added. After mixing, the cells were stained for 30 min under dark conditions at RT. Stained cells were immediately detected using a flow cytometer, and data were analyzed using FlowJo software 3.3.
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