To group isolates based on their epidemiological context, in silico MLST was performed using the Center for Genomic Epidemiology's MLST typing tool (https://cge.cbs.dtu.dk/services/MLST/). Clonal Complexes (CCs) were assigned based on the Pasteur Institute schema (http://www.pasteur.fr/recherche/genopole/PF8/mlst/Lmono.html). Novel sequence types (STs) were confirmed using Sanger sequencing and submitted to the Pasteur Institute Database for new assignments (http://bigsdb.pasteur.fr/listeria/listeria.html).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.