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Water Relations

AS Akanksha Sehgal
KS Kumari Sita
JK Jitendra Kumar
SK Shiv Kumar
SS Sarvjeet Singh
KS Kadambot H. M. Siddique
HN Harsh Nayyar
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Relative leaf water content (RLWC) was calculated according to the method of Barrs and Weatherley (1962). Leaf samples (4–5 leaves from top 2 or 3 branches) from each genotype were collected, weighed (fresh weight), immersed in distilled water for 3 h in a Petri dish, removed and weighed (turgid weight), and then oven-dried at 80°C for 24 h and reweighed (DW).

RLWC was calculated using formula:

Osmotic potential of the leaves was measured using an osmometer (Wescor, United States). ‘The stomatal conductance (gs) of fully expanded leaves (from the second or third branches from the top) was measured using a portable leaf porometer (model SC1, Decagon Devices, Pullman, WA, United States) at 11:00 h at the end of the stress period and expressed as mmol m-2 s-1′ (Awasthi et al., 2014).

Copyright and License information:  ©2017 Sehgal, Sita, Kumar, Kumar, Singh, Siddique and Nayyar.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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