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synNotch Driven Blinatumomab Production
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Primary human CD4+ T cells were transduced with the α-GFP nanobody (LaG17) synNotch Gal4VP64 receptor and 5× Gal4 response elements controlling α-CD19/CD3 BiTE, Blinatumomab expression. synNotch BiTE T cells were stimulated with either surface GFP+ or GFP- K562s for 24 hours and supernatant was harvested. The T cells were also collected and stained with α- CD69 APC (Biolegend #310910) to determine if they were activated.
Copyright and License information: ©2016
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