We tested cholinergic lesioned and control mice on the accelerating rotarod consisting of four trials per day for four consecutive days109. The rod, a mouse-specific apparatus accelerates from 5 to 40 rpm over 5 min and then remains at 40 rpm (revolutions per minute) for an additional 5 min for each trial. The latency time to fall from the rod was recorded up to a maximum of 10 min.
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