Mitochondrial respiration

ML Marion Laudette
YS Yannis Sainte-Marie
GC Grégoire Cousin
DB Dorian Bergonnier
IB Ismahane Belhabib
SB Stéphanie Brun
KF Karina Formoso
LL Loubna Laib
FT Florence Tortosa
CB Camille Bergoglio
BM Bertrand Marcheix
JB Jan Borén
OL Olivier Lairez
JF Jérémy Fauconnier
AL Alexandre Lucas
JM Jeanne Mialet-Perez
CM Cédric Moro
FL Frank Lezoualc’h
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Oxygen consumption rate (OCR) was measured with a Seahorse XFe24 analyzer (Agilent). ARVMs were seeded in 24-well Seahorse assay plates in complete medium and, after 2 h of cell attachment, the medium was replaced with XF basal medium supplemented with 10 mM glucose, 4 mM glutamine, and 1 mM sodium pyruvate (pH 7.4). Plates were incubated for an additional 1 h at 37 °C in a CO2-free incubator. The hydrated wells of the sensor cartridge were then loaded with 1 μM oligomycin (port A), 1 μM carbonyl cyanide 4- (trifluoromethoxy) phenylhydrazone (FCCP) (port B) and 1 μM antimycin A + 1 μM rotenone (port C). The data were analyzed using the Seahorse Wave software.

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