Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
An RNA extraction kit (Tiangen, Beijing, China) was used to extract the total RNA of carrot roots, leaf blades, and petioles according to the manufacturer’s instructions. cDNA was synthesized using a PrimerScript RT reagent kit (TaKaRa, Dalian, China). The cDNA was diluted 15-fold for quantitative reverse transcription PCR (qRT-PCR) analysis.
Copyright and License information: ©2017 Que, Wang, Xu, Wang and Xiong.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
