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To correct for egg chamber movement and/or growth during imaging, we measured border cell speed relative to the center of egg chamber by registering the outlines from frame to frame. We first used StackReg plugin (rigid body) in Fiji [ImageJ 1.49c; National Institutes of Health (46)] to align the egg chambers. Then we designed a Matlab code to further align the egg chambers (Supporting Information).
Copyright and License information: ©2016
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