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CT26 tumor cells were plated in triplicates in 60-mm dishes (Nunc Thermo Fisher, Waltham, MA, USA) at concentrations estimated to yield approximately 100 colonies/dish. Then, the cells were treated with irradiation of 1 × 5 Gy or 2 × 5 Gy. After incubation for approximately 2 weeks, the cells were fixed and adherent cells were stained with methylene blue (Sigma-Aldrich, Munich, Germany) for 30 min. Colonies with >50 cells were scored.
Copyright and License information: ©2017 Frey, Rückert, Weber, Mayr, Derer, Lotter, Bert, Rödel, Fietkau and Gaipl.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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