Whole Cell Patch Clamp and Two Electrode Voltage Clamp Electrophysiology

HEK293 cell electrophysiology was performed as previously described (Vafabakhsh et al., 2015). Drugs were applied using a gravity-driven perfusion system and illumination was controlled using a DG-4 (Sutter) in combination with excitation filters (379/34 and 500/24 nm for D-MAG-0, 445/20 nm for BGAG_12,460). See Supplementary Experimental Methods for details on oocyte two-electrode voltage clamp electrophysiology. Data was analyzed with Clampfit (Molecular Devices) and Prism (Graph pad).