Intravital real-time confocal laser-scanning microscopy

YA Y. Anraku
HK H. Kuwahara
YF Y. Fukusato
AM A. Mizoguchi
TI T. Ishii
KN K. Nitta
YM Y. Matsumoto
KT K. Toh
KM K. Miyata
SU S. Uchida
KN K. Nishina
KO K. Osada
KI K. Itaka
NN N. Nishiyama
HM H. Mizusawa
TY T. Yamasoba
TY T. Yokota
KK K. Kataoka
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The mice were anaesthetized with 2.5% isoflurane (Abbott, North Chicago, IL) using a Univentor 400 anaesthesia unit (Univentor; Zetjun, Malta). The skulls of mice were partially cut open around the ROIs without damaging blood vessels. The mice were directly placed on a thermoplate (Tokai Hit, Tokyo, Japan), and a cover slip (Muto Pure Chemicals, Tokyo, Japan) was attached with proper pressure to flatten the brain surface. Images of the micelle crossing the BBB were acquired with an A1R confocal laser-scanning microscopy system (Nikon Corp., Tokyo, Japan) attached to an upright ECLIPSE FN1 (Nikon Corp.).

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