Effect of phloretin on brain accumulation of nanocarrier

YA Y. Anraku
HK H. Kuwahara
YF Y. Fukusato
AM A. Mizoguchi
TI T. Ishii
KN K. Nitta
YM Y. Matsumoto
KT K. Toh
KM K. Miyata
SU S. Uchida
KN K. Nishina
KO K. Osada
KI K. Itaka
NN N. Nishiyama
HM H. Mizusawa
TY T. Yamasoba
TY T. Yokota
KK K. Kataoka
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To examine how the brain accumulation of the 25%Gluc(6)/m was effected by the inhibition of GLUT1, BALB/c mice (n = 4) were fasted for 24 h and then intravenously injected with the solution of the 25%Gluc(6)/m. After 30 min, they were intraperitoneally injected with 200 μl of 20 wt% glucose solution, followed by an intravenous injection of the GLUT1 inhibitor phloretin (10–50 mg/kg)38 at 20 min after glucose administration. The brain was extracted in the same manner as biodistribution study, and the accumulation of the 25%Gluc(6)/m in the brain was quantified by fluorescence measurement using the Infinite M1000 PRO (Tecan Group Ltd.).

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