To examine how the brain accumulation of the 25%Gluc(6)/m was effected by the inhibition of GLUT1, BALB/c mice (n = 4) were fasted for 24 h and then intravenously injected with the solution of the 25%Gluc(6)/m. After 30 min, they were intraperitoneally injected with 200 μl of 20 wt% glucose solution, followed by an intravenous injection of the GLUT1 inhibitor phloretin (10–50 mg/kg)38 at 20 min after glucose administration. The brain was extracted in the same manner as biodistribution study, and the accumulation of the 25%Gluc(6)/m in the brain was quantified by fluorescence measurement using the Infinite M1000 PRO (Tecan Group Ltd.).
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