Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Preparation and characterization of polymeric micelles

YA Y. Anraku
HK H. Kuwahara
YF Y. Fukusato
AM A. Mizoguchi
TI T. Ishii
KN K. Nitta
YM Y. Matsumoto
KT K. Toh
KM K. Miyata
SU S. Uchida
KN K. Nishina
KO K. Osada
KI K. Itaka
NN N. Nishiyama
HM H. Mizusawa
TY T. Yamasoba
TY T. Yokota
KK K. Kataoka
request Request a Protocol
ask Ask a question
Favorite

Detailed preparation schemes of the Gluc(6)/m, Gluc(3)/m and Null/m are given in the Supplementary Fig. 12 and Supplementary Table 1. The size distribution of the polymeric micelles was evaluated by conducting DLS measurements at 25 °C in 10 mM phosphate buffer at pH 7.4 using a Zetasizer Nano ZS90 (Malvern Instruments Ltd., Worcestershire, UK). TEM images of the polymeric micelles were obtained with a JEM-1400 (JEOL Ltd., Tokyo, Japan) according to the following sample preparation procedure: mesh copper grids were coated with a thin film of collodion followed by carbon coating, and a 0.1 mg/ml sample solution was placed on these carbon-coated grids. The sample on the grid was stained with 2 wt% uranyl acetate in a mixture of water and ethanol (v/v = 1/1) and then blotted to remove any extra stain.

Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A