4.8. Assessment of the Effect of p38 MAPK Overexpression on Cell Growth and Viability

The experiment was set up in the same way as that described in “p38 MAPK overexpression by plasmid transfection” with the following modifications. Cells were seeded at a concentration of 2 × 10⁴ cells/100 μL of relevant medium (see above) into the wells of the 96-well plate. The amount of plasmid DNA used was 100 ng per well. After 48 h of incubation with or without SA, the number of living cells was determined using a hemocytometer counting system after staining with trypan blue.