Autoaggregation and biofilm formation assay results were from a minimum of three independent replicates. The differences between the mean maximum percent autoaggregation due to temperature, redox balance, pH, blending, and various media; the differences between biofilm formation due to the presence of FliC were determined with SAS® (Version 9.4; SAS Institute) using the generalized linear model. When statistical significance was observed (P < 0.05), a post-hoc mean separation was run using Tukey's Honest Significance Difference test which controlled for unequal sample sizes. All data satisfied the assumptions of normality and homogeneity of variance.
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