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ECs were cultured under standard conditions to 80% confluence, serum starved for 24 hours, and then stimulated with MSC conditioned media (CM) from the various MSCs (healthy, ischemic, ischemic diabetic). Positive control cells were stimulated with complete media and PBS was added for a negative control. Cell growth was quantified (4 wells/time/analysis) after 24–48 h by Celltiter 96 Aqueous reagent and quantified by absorbance in microplate reader. Results were performed in triplicate and analyzed in a blinded fashion.
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