Mice.

Experiments were performed with C57Bl6J mice (Charles River Laboratories). One experiment (see Fig. 6) involved GP4.12 mice (Dana et al. 2014). Both male and female mice, between 8 and 22 wk of age, were included in studies. All surgery was performed under isoflurane anesthesia (1.25–2% vol/vol). Anesthetized mice were heated to maintain a body temperature of 37°C during all procedures.

Quantification of heating-induced damage. A: intensity of immunolabeling, as a fraction of intensity in contralateral hemisphere, in mice heated at different laser intensities, under continuous (20 min; ●) or intermittent illumination (1 h, 33% duty cycle, 10-s pulses; ○). Shaded area denotes the 95% confidence interval of the control group mean. B: intensity of immunolabeling, as a fraction of intensity in contralateral hemisphere, in GP4.12 (▼) or wild-type mice (●) heated at different laser intensities for 20 min. Shaded area denotes the 95% confidence interval of the control group mean. Control data (0 mW) are shared in A and B. *P < 0.05, multivariate analysis of covariance; n.s., not significant.