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Antisense oligodeoxynucleotide injections
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Antisense oligodeoxynucleotides targeting endogenous Msi1 and Msi2 mRNAs, Ringo mRNA or a randomized control oligonucleotide sequence 5′-TAGAGAAGATAATCGTCATCTTA-3′ were employed as previously described32, 53. Msi AS injected oocytes failed to undergo progesterone-stimulated maturation (GVBD) in the absence of expressed Msi protein. No inhibition of progesterone-stimulated maturation was observed with scrambled control antisense oligonucleotides.
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