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Bone marrow cells isolated from femurs and tibias from 7-week-old C57BL/6, IL-6−/−, IL-23−/−, and IL-17RA−/− naive mice were cultured (7 days, 37°C, 5% CO2) in RPMI 1640 medium supplemented with 20% FBS and 30% L-929 cell conditioned media, as previously described (46). After differentiation, cells were harvested and infected with P. brasiliensis (MOI of 0.04).
Copyright and License information: ©2017 Tristão, Rocha, Carlos, Ketelut-Carneiro, Souza, Milanezi and Silva.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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