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Expand Long Template PCR Buffer 1 (1×), dNTPs (200 µM; each, Invitrogen), semi-nested primer (0.4 µM) and Abridged Anchor Primer (0.4 µM, 5′ RACE System for Rapid Amplification of cDNA Ends, Invitrogen) (Supplementary Table S1) and Taq + Tgo polymerase blend (2.5 U, Expand Long Template PCR System, Roche) were combined with 10 µl of tailed product in a total volume of 50 µl. Amplification was achieved using the following cycling program: 94 °C for 2 min, 45 cycles of 95 °C for 10 sec and 59 °C for 6 min and a final extension of 72 °C for 5 min.
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