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Antibodies for flow cytometry on mouse cells

ST Sara Trabanelli
MC Mathieu F. Chevalier
AM Amaia Martinez-Usatorre
AG Alejandra Gomez-Cadena
BS Bérengère Salomé
ML Mariangela Lecciso
VS Valentina Salvestrini
GV Grégory Verdeil
JR Julien Racle
CP Cristina Papayannidis
HM Hideaki Morita
IP Irene Pizzitola
CG Camille Grandclément
PB Perrine Bohner
EB Elena Bruni
MG Mukul Girotra
RP Rani Pallavi
PF Paolo Falvo
EL Elisabeth Oppliger Leibundgut
GB Gabriela M. Baerlocher
CC Carmelo Carlo-Stella
DT Daniela Taurino
AS Armando Santoro
OS Orietta Spinelli
AR Alessandro Rambaldi
EG Emanuela Giarin
GB Giuseppe Basso
CT Cristina Tresoldi
FC Fabio Ciceri
DG David Gfeller
CA Cezmi A. Akdis
LM Luca Mazzarella
SM Saverio Minucci
PP Pier Giuseppe Pelicci
EM Emanuela Marcenaro
AM Andrew N. J. McKenzie
DV Dominique Vanhecke
GC George Coukos
DM Domenico Mavilio
AC Antonio Curti
LD Laurent Derré
CJ Camilla Jandus
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Murine ILCs were identified as Lineage (FITC-conjugated anti-CD3e (cn: 130-102-207, 1:200), anti-CD5 (cn: 130-106-202, 1:200), anti-CD11b (cn: 130-098-085, 1:200), anti-CD11c (cn: 130-102-466, 1:200), anti-CD19 (cn: 130-102-494, 1:200), anti-Ter119 (cn: 130-102-257, 1:200), anti-B220 (cn: 130-102-228, 1:200), anti-TCRγδ (cn: 130-109-796, 1:200), anti-DX5 (cn: 130-102-258, 1:200), anti-FcεRI (cn: 130-102-264, 1:50) (all from Miltenyi Biotec)) negative, CD127+ (eF660 anti-CD127 (clone: A7R34, 1:100, eBioscience)), CD45+ (PE-Cy7-conjugated anti-CD45 (clone: 30F11, 1:300, BioLegend)) lymphocytes. ILC subsets were identified using PE-conjugated anti-ST2 (cn: 145304, 1:100, Biolegend); Brilliant Violet 421-conjugated anti-NKp46 (cn: 130-102-185, 1:50, Miltenyi Biotec); PerCP-eFluor710-conjugated anti-cKit (clone: 2B8, 1:200, eBioscience).

Murine M-MDSCs were identified using PE-Cy7-conjugated anti-CD45 (clone: 30F11, 1:300, BioLegend), AlexaFluor700-conjugated anti-CD11b (clone: M1/70, 1:300, eBioscience) APC-eFluor780-conjugated anti-Gr1 (clone: RB6 AC5, 1:500, BD Pharmingen), APC-eFluor780-conjugated anti-Ly6C (cn: 47-5932-80, 1:600, eBioscience), PerCP-Cy5.5-conjugated anti-Ly6G (cn: 45-5931-80, 1:600, eBioscience) and PE-Cy7-conjugated anti-CD49d (clone 9F10, 1:200, BioLegend) antibodies, as previously described49, 50.

Additional antibodies used were Pacific Blue-conjugated anti-CD44 (clone: IM781, 1:100), Alexa647-conjugated anti-CD62L (clone: Mel14, 1:1000), TexasRed-conjugated anti-B220 (cn:551489, 1:50, BD Pharmingen) and Brilliant Violet 421-conjugated anti-SiglecF (cn: 565934, 1:200, BD Pharmingen) antibodies.

Copyright and License information: The Author(s) ©2017
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