Growth, Maturation, and Harvesting of C. elegans

Wild-type C. elegans were obtained from CGC and maintained on NGM at 20°C. The standard E. coli OP50 strain was used as a food source. Age synchronized young adult worms were used in all experiments. Synchronization was obtained through bleaching of gravid hermaphrodites to obtain eggs that were plated onto seeded plates. The young adult nematodes were harvested from NGM plates by using M9 buffer and used for experiments. Was grown on NGM agar plates and fed internationally accepted feed (the standard E. coli OP50 strain), as described previously (Ward, 1973). Standard techniques (Hope, 1999; Stiernagle, 1999) for the maintenance and growth of nematodes on NGM were used.