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At regular intervals (0, 30, 60, 90, 120, and 150 min) samples of B. subtilis wt were collected for cell density measurements. To immobilize cells for microscopy, NaN3 (7.7 mM final concentration) was added. The DIC microscopy counts were obtained utilizing Axio Observer Z1 microscope (Zeiss, Göttingen, Germany) with 20 × objective (NA 0.4 and 1.6 × optovar, Zeiss) and Neubauer improved bright-line grid counting chamber with the depth of 25 μm (Brand, Germany). For each time point four chambers were manually evaluated by counting cells in the entire grid.
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