Haemoglobinopathies and β-thalassaemia were assessed using the β-thalassaemia Short Program from the Variant Haemoglobin Testing System® (Bio-Rad, Hercules, USA). Detection of α-thalassaemia (3.7 kb deletion) was performed by the GAP-PCR method [20]. Glucose 6-phosphate dehydrogenase (G6PD) deficiency was determined using the Beutler fluorescent spot test [21].
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