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Measurement of the plasma digoxin levels
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Twenty‐four hours after the final injection, blood was collected from the retro‐orbital vein, mixed and drawn into a capillary tube. Plasma was separated from the blood samples via low‐speed centrifugation (3000× g for 10 min at 4°C), collected and frozen at −80°C until analysis. The plasma digoxin levels were measured using a commercially available kit (Monobind Inc. Lake Forest, CA 92630, USA) according to the manufacturer's instructions.
Copyright and License information: ©2016 The British Pharmacological Society
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