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BRAF and RAS Mutation Analysis
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DNA was isolated from FNA samples using the QIAamp DNA Micro kit (Qiagen, Italy) according to the manufacturer’s protocol. The BRAF (NM_004333.4) status of exon 15 was assessed by direct sequencing. The primers and PCR reaction protocol have been described elsewhere (14). Exons 2 and 3 of H-RAS (NM_005343.2), K-RAS (NM_033360.2), and N-RAS (NM_002524.3) were analyzed by direct sequencing, as described elsewhere (15, 16).
Copyright and License information: ©2017 Censi, Cavedon, Bertazza, Galuppini, Watutantrige-Fernando, De Lazzari, Nacamulli, Pennelli, Fassina, Iacobone, Casal Ide, Vianello, Barollo and Mian.
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