According to official information from the Wuhan Animal Health Inspection Institute, China, there are 89 veterinary clinics and hospitals in Wuhan, China. In the present study, a total of 118 blood samples from pet dogs were randomly collected from five clinics with the permission of the dogs' owners. All samples were screened via both microscopic examination and reverse line blot (RLB) hybridization assays at the College of Veterinary Medicine of Huazhong Agricultural University.
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