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Viruses and Cells

XH Xiaoli Hao
JW Jiongjiong Wang
JH Jiao Hu
XL Xiaolong Lu
ZG Zhao Gao
DL Dong Liu
JL Juan Li
XW Xiaoquan Wang
MG Min Gu
ZH Zenglei Hu
XL Xiaowen Liu
SH Shunlin Hu
XX Xiulong Xu
DP Daxin Peng
XJ Xinan Jiao
XL Xiufan Liu
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Four H5 HPAIV strains including A/chicken/Anhui/QD1/2014 [QD/1 (H5N1)], A/goose/Jiangsu/QD5/2014 [QD/5 (H5N8)] (Li et al., 2016), A/chicken/Jiangsu/YB7/2015 [YB/7 (H5N1)] and A/Chicken/Jiangsu/k0402/2010 [CK/10 (H5N1)] (Hu et al., 2013), and one genotype S H9N2 virus A/Chicken/Jiangsu/CZ73/2014 [CZ/73 (H9N2)] were used in this study. QD/1 and QD/5 belong to subclade 2.3.4.4 while YB/7 and CK/10 belong to subclade 2.3.2.1c. The viruses propagated in 9- to 10-day-old specific-pathogen-free (SPF) chicken embryos.

Chicken embryo fibroblasts (CEF), and chicken fibroblast cell line (DF-1) cells were cultured in DMEM (Invitrogen) medium with 4% fetal bovine serum (FBS) (Gibco). Madin-darby canine kidney (MDCK), and human embryonic kidney (293T) cells were grown in DMEM containing 10% FBS. All cells were incubated at 37°C with 5% CO2. For virus inoculation experiments, DMEM medium supplemented with 1% FBS was used.

Copyright and License information:  ©2017 Hao, Wang, Hu, Lu, Gao, Liu, Li, Wang, Gu, Hu, Liu, Hu, Xu, Peng, Jiao and Liu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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