All experiments involving live viruses were carried out in a biosafety level 3 (BSL3) facility at Yangzhou University in accordance with the institutional biosafety manual.
Animals were housed in negative-pressure isolators with HEPA filters in a BSL3. The protocols for animal experiments were approved by the Jiangsu Administrative Committee for Laboratory Animals (approval number: SYXK-SU-2007-0005), and complied with the guidelines of Jiangsu laboratory animal welfare and ethics of Jiangsu Administrative Committee of Laboratory Animals.
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