Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Statistical Analyses

VK Varun Kumar
AB Ankush Bansal
RC Rajinder S. Chauhan
request Request a Protocol
ask Ask a question
Favorite

The one and two-way analysis of variance (ANNOVA) followed by a Bonferroni test were performed from data in triplicates (mean ± SD) using GraphPad prism software version 6.0. To examine the correlations between gene expression profiles obtained from RNA-seq and qRT-pCR, log2 fold change values were estimated among different shoots and stolons accessions. The scatterplots were then created by comparing log2 fold change values determined by RNA-seq and qRT-PCR using GraphPad prism software version 6.0. The correlations were represented in terms of coefficient of determination, i.e., R2.

Copyright and License information:  ©2017 Kumar, Bansal and Chauhan.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A