The annotation and statistical analysis of each transcript was performed by homology based method against NCBI nrdb protein database using Blast2GO software through Blastx module (Conesa et al., 2005). The parameters used for this analysis were; (a) e-Value b = 10-e4, (b) Similarity ≥35%, (c) Annotation cutoff ≥55, (d) GO weight cutoff ≥5. Moreover, domain level annotation was also performed by using the Online InterProScan tool RunIprScan-1.1.01 (Henschel et al., 2012).
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