For drug and stress sensitivity assays, 5 × 104 conidia from NK17, Δgcn2, and the complementation strain Δgcn2::GCN2 were incubated on PLA plates separately supplemented with 0.02% Congo red, 0.5 M KCl, 1 M NaCl, 2 mM sorbitol, 2 mM NaNO2, 100 μg/mL G418, 4 μg/mL nystatin and 50 ng/mL calcofluor white. All cultures were incubated at 28°C for 7 days.
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