UV-Vis spectroscopy studies

Measurements for GSH-mediated Cleavage of AzoDiTAB were performed on a Shimadzu UV-2550 UV-Vis spectrophotometer using trace quartz cuvettes (d = 10 mm). The spectra signals were recorded in the 240–500 nm wavelength range at a middle speed. 50 μM AzoDiTAB was incubated with 10 mM or 0.5 mM GSH, and the incubation samples were detected in a time range from 0 h (control) to 107 h. Before the measurement, the signal of the buffer was subtracted from the spectra of the samples.