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Apoptotic cells in kidney sections were detected using the One-Step TUNEL Apoptosis Assay Kit (Beyotime Biotechnology, China) according to the manufacturer's instructions. Briefly, the tissues were dehydrated, transparentized, impregnated, and embedded. Sections were then incubated with 3,3-diaminobenzidine (DAB), and the brown particles in the nucleus indicated positive staining.
Copyright and License information: ©2021 Xiao-lu Wang et al.
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