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Microscopy

BG Bernhard Gillissen
AR Antje Richter
FE Frank Essmann
WK Wolfgang Kemmner
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Cancer cells were seeded into 6-well plates (2 × 105 cells/2 ml), treated for 24 h with 10 μM Crizotinib or Alectinib. Thereafter, cells were incubated with 100 μM ALA for 4 h. Microscopy images were then taken using an Axiovert 200 inverse fluorescence microscope (Carl Zeiss, Inc.) equipped with a Hamamatsu ORCA-ER digital camera using the Openlab software (Improvision). Mean fluorescence intensity of images was analyzed using ImageJ 1.53a software (https://imagej.nih.gov/ij/).

Experimental data were presented as the mean ± standard deviation (SD). Statistical analyses were performed using the GraphPad Prism 5. Student t-test was used for data analysis between two groups. P-values of less than 0.05 were considered statistically significant.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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