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Correlative Light and Electron Microscopy (CLEM)
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Cells were grown on gridded glass coverslips (MatTek Corporation, Ashland, MA). Immunofluorescence staining was performed as described above, following which cells were refixed with 2% PFA, 2.5% glutaraldehyde, 0.1 M cacodylate and processed for conventional EM. Resin stubs were inverted over areas of interest and baked overnight. Coverslips were subsequently removed using liquid nitrogen.
Copyright and License information: ©2016, Edgar et al
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