2.9. Pyloric ligation-induced ulcer model

Forty eight fasted (for 24 h) male Swiss albino mice were randomly assigned to eight different groups (1–8, n = 6). Group 1 (negative control) received distilled water; Group 2, 3, and 4 were treated with 100 mg/kg, 200 mg/kg and 400 mg/kg of the leaf extract, respectively; Group 5, 6 and 7 were treated with 100 mg/kg, 200 mg/kg and 400 mg/kg of the root extract, respectively; and Group 8 was treated with the standard drug (Ranitidine, 50 mg/kg). Pylorus ligation was performed in all groups of fasted mice to induce gastric ulcer 1 h after the administration of treatments [29,30].

Male mice were randomly assigned to eight groups (1–8, n = 6) and treated as mentioned in the single dose study. Treatments were administered daily at morning for 10 days. The animals were fasted for 24 h before administration of the 10th day treatment, and pylorus ligation was done 1 h after the administration of treatments [29,30].