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2.2. Genomic DNA and Plasmid DNA Extraction

MI Muhammad Hafiz Ismail
KM Katharine A. Michie
YG Yu Fen Goh
PN Parisa Noorian
SK Staffan Kjelleberg
ID Iain G. Duggin
DM Diane McDougald
SR Scott A. Rice
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Genomic DNA was extracted using the QIAamp® DNA Mini Kit (Qiagen, Germany), while plasmid DNA was extracted with the FavorPrep plasmid extraction kit (Favorgen, Taiwan) as per the manufacturers’ instructions. The concentration was assessed using both a NanoDrop 2000 Spectrophotometer (Thermo Scientific, MA, USA) and Qubit® 2.0 Fluorometer (Life Technologies, CA, USA).

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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