On day 15 (euthanasia), radiographic images of the left tibia from each mouse were obtained using an LX-60 Faxitron laboratory radiography system (Faxitron X-ray Corp., Wheeling, IL, USA). The tibias were imaged at 28 KV for 5 s to qualitatively evaluate the effect of SP600125 on bone tumor growth in mice bearing Ace-1-Dkk-1YFP-Luc intratibial tumors.
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