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HPLC analysis was performed in a Varian 920-LC Liquid Chromatograph (Varian Inc, Walnut Creek, CA, USA) with a built-in auto-injector and UV-Vis detector. Kinetex C18 column (100 mm × 4.6 mm, 2.6 μm), (Phenomenex, Torrance, CA, USA) was used, with a flow rate of 1 mL/min; mobile phase A of 0.1% acetic acid in water; mobile phase B of acetonitrile; linear gradient of 0 to 8 min, 0–85% of B. The HPCL separation was monitored by the UV-Vis detector at 260 nm.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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